SR9216 - Metaproteomic Analysis
for Identifying and Engineering of Porcine Gut Symbiotic Bacterial Enzymes
for Lignocellulose Degradation
This project was funded by OMAFRA through the 2009 New Directions
& Alternative Renewable Fuels Research program.
Lead researcher
Dr. Ming Fan,
Dept. of Animal and Poultry Science, University of Guelph
Objectives
- To identify novel porcine gut symbiotic bacterial hemicellulases,
i.e., arabinanases and xylanases, and cellulases for specifically
processing typical Ontario agricultural waste feedstock such as
corn stalks, soybean stover and distillers dried grains with soluble
(DDGS) through metaproteomic analysis.
- To perform enzymatic, biochemical and molecular characterization
of identified target enzymes for understanding their catalytic
activities, pH optimum, thermostability and structure-function
relationships.
- To conduct genetic engineering for enzyme optimization through
mutagenesis and over-expression of identified target hemicellulase
and cellulase enzyme proteins in bacteria and fungi using commercial
vectors.
- To pursue commercialization and application of studied target
novel hemicellulase and cellulose enzymes as industrial enzymes
with industrial partners and our institutional business offices
in Ontario, Canada and the world.
Expected benefits
Potential benefits arising from novel hemicellulases and cellulases to
be developed and commercialized from this proposed project include:
- development of lignocellulosic bioethanol-based bioeconomy;
- improving sustainability of Ontario livestock industries;
- improving Ontario economic viability especially in the rural
community by increasing profit margins associated the agricultural
production;
- training of highly qualified personnel (HQP) and developing
effective collaborative research capacity on agricultural biotechnology
and industrial enzyme development in Ontario.
Results
Currently not available
Related information
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