2004 Compendium of OMAFRA Funded Environment Research:
Water Quality (WQ)

| 2004 Compendium of OMAFRA Funded Environment Research - Index Page |

WQ1 Development of Alternative Irrigation Practices to Improve Water-Use Efficiency, Nutrient-Use Efficiency and Water Quality

Project Leader: Ron Beyaert
Southern Crop Protection and Food Research Centre
Agriculture and Agri-Food Canada
Delhi ON N4B 2W9
519-582-1950 x240
beyaertr@agr.gc.ca

Collaborating Researchers: B.R. Ball Coelho and R.C. Roy, Agriculture and Agri-Food Canada

Project Duration: June 2002 - June 2004

Project #: SR9119

Abstract:
The development of a research program to investigate the potential for surface (DI) and subsurface drip irrigation (SDI) on coarse-textured soils is necessary to better utilize irrigation water while reducing environmental impact in the production of high value crops. This project will complete a field trial to assess the ability of alternative irrigation/fertilization technologies to increase water and nutrient use-efficiency in the production of high-value crops. The use of micro-irrigation technology may allow more efficient use of Ontario irrigation water resources while reducing the potential for leaching of nutrients, particularly nitrogen, into the groundwater. This will be accomplished by applying these nutrients only to the zone of soil containing the bulk of the plant roots, in small amounts, and only as required by the crop through the irrigation system.

The overall objective of the study will be met by determining yield, quality, nutrient uptake and leaching losses of both once-over harvested and multi-pick cucumber cultivars under various irrigation/fertilization schemes in field plots. A total of 7 treatments will be evaluated. Surface drip and subsurface drip (20 cm deep) irrigation treatments will be compared to conventional dry fertilizer application at recommended application rates with overhead irrigation. Each drip irrigation treatment will have 3 levels of fertilizer application below, at and above OMAF recommended application rates. Irrigation scheduling will be based on soil moisture determinations.

Crop production systems that incorporate these irrigation technologies are expected to increase water-use efficiency through reduced losses to evaporation, improved scheduling and greater capacity for control of internal drainage and reduce nutrient loss to the environment through reduced leaching losses.

WQ2 Production/management of floriculture greenhouse crops

Project Leader: Theo J. Blom
Plant Agriculture
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x53847
tblom@uoguelph.ca

Project Duration: May 2002 - April 2006

OASIS #: 25863

Abstract:
The floriculture research program is directed at solving the needs of the floricultural production industry with applied research objectives.

The projects involve a comprehensive research program to improve various production management techniques for both cut flowers and potted plants through 5 objectives:

  • plant modifications through light quality, temperature manipulation, water temperature, thygmo morphogenesis or other non-chemical means.
  • more efficient use of production inputs such as energy, light, carbon dioxide and temperature control.
  • more sustainable production through optimizing nutrients and water through recirculation and disease management.
  • improvement of increased acceptability through species/cultivar selection or improved post harvest quality of potted plants or cut flowers

WQ3 Reduction of solid and phosphorus waste outputs of salmonids through improvement of feed formulation

Project Leader: Dominique Bureau
Animal & Poultry Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x53668
dbureau@uoguelph.ca

Project Duration: May 2001 - April 2004

OASIS #: 25673

Abstract:
The environmental impact of fish culture is becoming a matter of concern for the public and various levels of government in Ontario. Solid organic matter wastes settling to the sediment can negatively impact freshwater ecosystems by creating hypoxic conditions. Phosphorus (P) waste output is also a concern, since P is generally the most limiting factor for algae growth in freshwater, and excess P may lead to eutrophication of the receiving water body. Reduction of outputs from these two types of waste is key to ensuring the long-term sustainability of salmonid culture in Ontario.

Since these two types of wastes are ultimately from dietary origins, efforts to reduce them should be at the source, namely the feeds. Very significant reductions in solid organic matter and P waste outputs have been achieved by aquaculture operations over the past three decades through use of improved quality feeds. However, more progress can be achieved through fine-tuning of the feed composition, the use of new or improved ingredients, and the use of dietary additives to increase the digestibility of organic matter and P of common feed ingredients.

This project will involve a series of trials to explore the effect of various feed formulae based on novel or under exploited economical ingredients, and dietary additives (exogenous enzymes) on growth performance and solid and P waste outputs of rainbow trout. In addition, methods to reliably assess physical characteristics (cohesiveness, density) of waste produced by fish will be developed. The information generated by this project will be presented to stakeholders of the industry during various meetings and workshops and through technical and scientific publications.

WQ4 (AQ, SQ) Conflict resolution in rural Ontario: Responding to the intensification of agriculture

Project Leader: Wayne Caldwell
Environmental Design & Rural Development
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x56420
wcaldwel@rpd.uoguelph.ca

Collaborating Researcher: Stewart Hilts, Land Resource Science

Project Duration: May 2003 - April 2005

OASIS #: 25974

Abstract:
This research has the objective to identify best practices in the management of conflict associated with the intensification of agriculture. It shall apply principles of community development to enhance capacity to address issues of environmental and rural sustainability. In this context the research will be of assistance to communities, farmers, farm organizations, planners, policy makers and local government.

Issues related to agricultural intensification including odour, water quality, and water usage can create animosity within the community. The management of this conflict is required to minimize the threat to agricultural production from poor community relations. This conflict can often be resolved, but requires skilled facilitation to encourage discussion, understanding and in turn resolution of problems.

This research will evaluate approaches used beyond Ontario. In Manitoba, for example Manitoba Pork Producers have initiated a program of peer review mediation and in Alberta the Agricultural Operations Act provides opportunities for mediation. Ontario's recently adopted Nutrient Management Act contemplates the establishment of local committees to respond to conflict.

The research will deliver the following tangibles:

  • The research will monitor different approaches and the success of local conflict resolution and identify best practices.
  • The experience of the farm community with the Ontario Municipal Board, the Normal Farm Practices Protection Board and the courts will be documented and analyzed.
  • The research will evaluate the opportunity for local committees to assist in mediating disputes.
  • A manual will be prepared to assist local initiatives to establish committees to mediate agricultural disputes.

WQ5 The development of DNA microarray technology for use in testing of key microbial contaminants in water samples

Project Leader: Shu Chen
Laboratory Services Division
University of Guelph
Guelph ON N1H 8J7
519-767-6319
schen@lsd.uoguelph.ca

Collaborating Researchers: M. Griffiths, Department of Food Science; K. Rahn, Health Canada; B. Brooks, Canadian Food Inspection Agency; R. Fayek, Virtek Vision

Project Duration: October 2001 - January 2003

Project #: SR9087

Abstract:
The key contaminants in surface and ground water resources, including Cryptosporidium, Giardia, Cyclospora, Escherichia coli, Salmonella, Listeria, and Campylobacter, are common causes of waterborne diseases. Rapid, simple and cost-effective detection of these key pathogenic contaminants is crucial in ensuring water safety and a healthy environment. The purpose of this research was to develop a practical DNA microarray for use in diagnostics and water or food safety testing.

This project was a continuation/extension of previous and ongoing research on the development of a DNA microarray-based test for detecting key food-borne bacterial pathogens including Shiga toxin producing Escherichia coli (STEC), E. coli serotype O157:H7, Campylobacter, Salmonella, Salmonella typhimurium DT104, and Listeria monocytogenes. In this project, we expanded the pathogen coverage on the bacterial microarray to include key waterborne parasites: Cryptosporidium parvum, Giardia intestinalis and Cyclospora cayetanensis, and developed a fluorescent multiplex PCR assay capable of simultaneously amplifying the specific genes of the three parasites. The functionality of the expanded microarray and associated procedures in testing the nine pathogens in pure cultures as well as in spiked water samples was demonstrated.

The multiplex PCR-microarray system was evaluated for its specificity using 30 parasitic strains, 60 target bacteria strains and 52 non-target bacterial and parasitic strains. All of the confirmed target strains were correctly detected; all of the non-target strains were not detected. The system was able to detect approximately 50-100 copies of template DNA in pure cultures and about 100 copies of template DNA in spiked water sediment samples for the targeted parasites. The system was further evaluated using 30 spiked water sediment samples that contained one, two, three or four pathogens together in one sample, and resulted in correct matches between the microarray results and the identities of the samples.

It was concluded that the expanded multiplex PCR-microarray system developed in this project is capable of detecting the three parasitic and the six bacterial pathogens in a single assay. It should potentially be a valuable tool for comprehensive and cost-effective water/food safety testing. Further improvement in the limit of detection of the assay and more validation studies using naturally contaminated water samples are suggested before the system can be implemented in diagnostic laboratories.

WQ6 (SQ) Efficient and environmentally sound use of nitrogen fertilizers, organic amendments, irrigation and herbicides in Ontario orchards

Project Leader: John Cline
Plant Agriculture
University of Guelph
Simcoe ON N1G 2W1
519-426-7127 x331
jcline@uoguelph.ca

Project Duration: June 2002 - June 2005

Project #: SR9110

Abstract:
There is increasing demand in modern high density tree fruit orchards to intensify the use of nitrogenous fertilizers, herbicides, and supplemental irrigation water to maximize precocity and fruit quality. Increasing globalization and decreasing profit margins, coupled with the rising consumer demand for safe food produce with less chemical inputs, is creating uncertainty and instability for Ontario producers with these two seeming opposing pressures.

The overall goal of this project is to provide a research database and ultimately give fruit producers the means to reduce chemical and water inputs without sacrificing fruit quality or profitability. This research will specifically address the efficient and safe use of nitrogen (N) fertilizers, organic amendments, irrigation and herbicides. The apple orchard will be used as a model, however, the results will be directly applicable to other tree fruit species, such as peaches or pears grown under similar management practices in Ontario.

The specific objectives will be to: a) investigate the efficiency of N uptake and fate of N in the soil and groundwater; b) investigate new subsurface drip micro irrigation technology for improved water and nutrient use efficiency, groundwater protection and improved weed control and to compare this with standard trickle irrigation best management practices currently being recommended; c) investigate the benefits of various organic amendments and alternative non-chemical forms of weed control for increasing tree establishment, tree vigour, precocity and yield of tree fruit species; d) perform an economic cost benefit analysis associated on same; e) quantify the environmental benefits on the same; f) measure existing and alternative chemical-free forms of weed control on soil water quality, water use, plant water use efficiency, and; g) determine the effects of organic and inorganic soil amendments on selected soil biological, chemical, and physical properties indicative of soil quality.

WQ7 Environmentally friendly nursery production research

Project Leader: Calvin Chong
Plant Agriculture
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x53032
cchong@uoguelph.ca

Collaborating Researcher: Glen Lumis, Plant Agriculture

Project Duration: May 2002 - April 2006

OASIS #: 25758

Abstract:
The ornamental nursery/landscape industry ($355 million for nursery stock and sod, and $4 billion in annual landscape upgrades) has traditionally been one of the fastest growing agricultural sectors in Ontario. However, increasing concerns over the disposal of wastes and water quality have heightened the significance of our "environmentally-friendly" research program. Our program is focussed on nutrient leachate recycling and the utilization of waste products and garbage-derived composts for use in growing ornamentals.

This research program looks at aspects of nursery culture with emphasis on container-growing and introduction of new or innovative environmentally- friendly practices. In view of the recent introduction of Ontario's Nutrient Management Act, the results are anticipated to have a large and beneficial impact on the nursery industry. This research will help to develop and fine-tune the needed wastewater and nutrient recycling technology and have it ready for producers to apply into commercial practice.

WQ8 Development of integrated control systems and management strategies for nutrient control in closed fertigation systems

Project Leader: Michael Dixon
Environmental Biology
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52555
mdixon@uoguelph.ca

Collaborating Researcher: Bernard Grodzinski, Plant Agriculture

Project Duration: May 2002 - April 2006

OASIS #: 25853

Abstract:
Issues surrounding water and fertilizer usage, continue to be of importance to Ontario's greenhouse industry. Closed loop fertigation systems are gaining greater acceptance by the greenhouse industry. However there are still several key problems (diseases, nutrient ion imbalances, salt accumulations and growth media waste) that growers must face when adapting this technology.

Past work has shown that closed fertigation systems can lead to ion imbalances if based solely on EC and pH technologies. There is a need for the development of fertigation systems that are based on individual ion management. This system will allow individual, real-time control of fertigation and will be based on nutrient modeling and sensor technology. Models of nutrient uptake by crops can serve the purposes of real-time control and prediction as part of an integrated control system with sensors. The research will also examine the use of copper and ozone as remediation technologies for nutrient solutions and growth media, and low nutrient strategies as a method of reducing salt accumulations in sub-irrigated systems and nutrient discharge.

WQ9 Quantifying water quality impacts of livestock farms and other rural land uses

Project Leader: Ron Fleming
Ridgetown College
University of Guelph
Ridgetown, ON N0P 2C0
519-674-1612
rfleming@ridgetownc.uoguelph.ca

Project Duration: May 1999 - April 2003

OASIS #: 23770

Abstract:
This study attempted to put into perspective the various sources in a watershed of water contaminants. It examined microbiological indicators, such as bacteria, Cryptosporidium, and Giardia. It established typical impacts of a variety of agricultural practices. Factors such as type of tillage, crop fertility program, livestock manure spreading, and type of crop grown all can potentially affect water quality. The study went further, though, and determined the potential impacts of other land uses in the watersheds. Often, agriculture is assumed to be the largest contributor of contaminants in a rural watershed, since it has the largest land area. There are other impacts, however, that are not well documented - for example: illegal connections of septic systems to drainage tiles, sewage treatment plant discharges of raw sewage, and wildlife impacts.

Manure and drainage water samples were analyzed for levels of Cryptosporidium and Giardia. This helped show the potential for manure management practices to impact levels of these contaminants in surface water, compared to other typical land uses in a watershed.

The second part of the study dealt with the manure volumes on swine farms using wet/dry feeders. This feeding system reduces water wasted in a pig barn. This can have a major impact on farmers using nutrient management planning to determine manure spreading rates. This, in turn, can have an impact on the potential for contamination of surface water or groundwater. Several farms were being monitored to determine manure production rates and manure nutrient concentrations under the two management practices used on most swine farms. Water needs of the pigs were also being measured.

WQ10 Development of Best Management Practices to protect rural water quality

Project Leader: Ron Fleming
Ridgetown College
University of Guelph
Ridgetown, ON N0P 2C0
519-674-1612
rfleming@ridgetownc.uoguelph.ca

Project Duration: May 2003 - April 2007

OASIS #: 26004

Abstract:
This project aims to measure the impact of a variety of farm practices on water quality (both surface water and groundwater) with the goal of developing Best Management Practices for the protection of the resource.

It will also verify the effectiveness of existing BMP's. Areas of focus will include: dead stock disposal, tile drain water quality, liquid manure storage and handling, and nutrient management planning. Parameters of greatest concern will be nutrients and pathogens.

WQ11 The effect of nutrient availability on biogeochemical processes affecting the transport of Escherichia coli in soils

Project Leader: Danielle Fortin
Department of Earth Sciences
University of Ottawa
Ottawa ON K1N 6N5
613-562-5800 x6423
dfortin@science.uottawa.ca

Project Duration: September 2001 - September 2003

Project #: SR9080

Abstract:
Escherichia coli is a pathogenic bacterial species associated with manure. As large quantities of manure are produced by the pork and cattle industries, and because the manure is susceptible to leaching by rain and surface water, E. coli is a common groundwater contaminant in rural Ontario (e.g. Walkerton). An ability to predict the speed and extent of E. coli transport through soils is therefore essential to evaluate the environmental risks associated with pork and cattle production in Ontario, and, where required, to facilitate environmental remediation.

The transport of bacteria through soil is governed by several hydrological and biogeochemical processes. Several recent studies have investigated the hydrological processes affecting bacterial transport in soils (e.g. groundwater flow, moisture content, change in hydraulic conductivity due to clogging of pores by bacteria). Biogeochemical processes, however, are presently poorly understood. Adhesion to minerals is the most important biogeochemical process affecting bacterial transport through soils. Bacteria-mineral adhesion is controlled by many intricately interrelated variables, including pH, ionic strength, solution composition, mineralogy, bacterial growth phase and metabolic activity. Moreover, our preliminary research indicates that the availability of nutrients (such as carbon, phosphorus and nitrogen, as would also be supplied to soil by leaching of manure) can affect many of these controlling variables, thereby indirectly affecting bacterial-mineral adhesion, and, ultimately, controlling bacterial transport through soils.

This investigation will conduct batch experiments to determine the degree of E coli K-12 adhesion to soil minerals as nutrient availability, solution pH and soil mineralogy are independently varied. The hypothesis is that nutrient limitation will affect cell size, surface electric charge, hydrophobicity and metabolic state. Nutrient concentrations will also affect solution composition and ionic strength. This project predicts that these changes in cell morphology, reactivity and solution composition will affect the extent to which the cells adhere to different mineral surfaces.

The major deliverable of this project will be a predictive model that can be applied to determine the degree of bacteria-mineral adhesion in a variety of different biogeochemical regimes, and thus be used to evaluate the environmental risks associated with manure leaching. The use of this sort of predictive tool will not only allow us to reduce the risk of environmental contamination in Ontario, but it will also provide an economic benefit, in that costs associated with site remediation will be lessened.

WQ12 (SQ) Evaluation of the impact of antibiotics on the survival, and surfactants on the transport of manure bacteria after land application

Project Leader: Michael Goss
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52491
mgoss@uoguelph.ca

Collaborating Researcher: Paul Sibley, Environmental Biology

Project Duration: May 2002 - April 2003

OASIS #: 25888

Abstract:
A previous study showed that the hydrophobicity of the bacterial cell was the most important character for bacteria retention to surfaces. This character is largely independent of the electrochemical properties of the suspending solution. The bacterial cell surface acts as an amphiphilic surface where both non-ionic and ionic moieties occur. Most of the charges on the wall are negative and therefore act as cation exchange sites, similarly to the clay-organic complexes.

Charges on the surfaces tend to be neutralized by the hydrated free ions present in the suspending solution. However organic cations tend to displace these inorganic ions thereby transforming the former hydrophilic sites in organophilic (hydrophobic) loci. Organic matter in suspension can interact with both ionic and non-ionic moieties.

Cationic surfactants can increase capacity of soils to absorb hydrophobic molecules. They are retained at cation exchange sites, such as those on clays and bacterial cell walls, imparting them a hydrophobic character.

We hypothesized that increasing the hydrophobicity of the soil and bacterial cells, by addition of cationic surfactants, would result in increased adsorption of bacterial cells to the soil particles and thereby reduce the likelihood of transport to groundwater.

Subtherapeutic use of antibiotics in feed rations is a common method for preventing disease and improving feed efficiency. However, very little information is available on their presence in manure and their ecotoxicological effects.

This study determined manure residue levels and the longevity of bacteria exposed to two antibiotics, tylosin and monensin, commonly used subtherapeutically.

WQ13 (SQ) Reduction of the survival and transport of bacteria after land application of organic materials contaminated with microbial pathogens

Project Leader: Michael Goss
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52491
mgoss@uoguelph.ca

Collaborating Researchers: P. Sibley, Environmental Biology; L. Evans, Land Resource Science; B. Van Heyst, School of Engineering

Project Duration: May 2003 - April 2005

OASIS #: 26074

Abstract:
The potential for pathogens to contaminate water resources after the land application of organic amendments, such as manure and sewage biosolids, depends on their ability to survive in soil. Previous work has shown that survival differs according to the properties of the receiving soil, the material containing the pathogens, and the characteristics of the microbes. The extent to which survival depends on the composition of the amendment, and the competition between the introduced microbes and the indigenous soil population is largely unknown. Criteria has been established for better management practices that reduce the survival and hence the risk to water resources from pathogens.

The ammoniacal nitrogen content of organic amendments varies, depending on the storage and processing undertaken, or the addition of bedding material in the case of manure. This research will investigate how this variation affects the survival of enteric microbes by the addition of urea to the amendments and to mixtures with different soils, and monitoring the survival of enteric microbes over a period of up to 6 months. The pH of the soil becomes more acidic as ammonia is lost by volatilization. Changes in pH can alter the point of zero charge of the surface of organisms, making them more or less likely to adhere to soil particles. A major objective is to model the ammonia content of the mixtures with time, taking account of the changes in pH and losses by volatilization. The project will also examine the significance of antimicrobial incorporation into the feed of cattle.

WQ14 Integrated management of aerial root environments in greenhouses - Recirculating hydroponic systems

Project Leader: Bernard Grodzinski
Environmental Biology
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x53439
bgrodzin@uoguelph.ca

Collaborating Researcher: Michael Dixon, Plant Agriculture

Project Duration: May 2002 - April 2006

OASIS #: 25879

Abstract:
This project focuses on production problems in closed environments and integrates analyses of plant physiology and epidemiology. The project examines re-circulation of nutrient media in hydroponically grown greenhouse crops and tests similar systems for manned space programs to provide food, oxygen, and potable water. Physical re-mediation technologies, such as ultraviolet radiation (UV), to kill pathogens in recirculating nutrient solutions, are linked with epidemiology studies testing the efficacy of biocontrol agents.

New non-invasive methods of monitoring canopy health, predicting and controlling the disease are being tested. This project complements other related studies on closed fertigation systems and ion imbalances, efficacy of new biocontol agents, and improvement of plant productivity by selection of new plant lines (e.g., those with low-light tolerance).

WQ15 Nitrate leaching in a clay loam soil after 44 years of consistent soil and crop management

Project Leader: Pieter Groenevelt
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120
pgroenev@lrs.uoguelph.ca

Collaborating Researchers: H. Dadfar, Land Resource Science; C. F. Drury, W. D. Reynolds and C. S. Tan Agriculture and Agri-Food Canada

Project Duration: May 1999 - April 2003

OASIS #: 23890

Abstract:
Management of water resources in areas susceptible to nitrate contamination can benefit from knowledge of long-term, or "baseline", nitrate leaching losses below the crop root zone. The objective of this study was to characterize nitrate loss from twelve tile drained plots on a Brookston clay loam soil (Orthic Humic Gleysol) which have had consistent agricultural management for forty-four years. The treatments included conventionally tilled fertilized and unfertilized continuous corn (Zea mays L.), continuous Kentucky bluegrass (Poa pratensis L.), and a conventionally tilled corn-oats (Avena sativa L.)-alfalfa (Medicago sativa L.)-alfalfa rotation with each crop in the rotation grown every year. Tile discharge, nitrate concentration in tile drainage water, and total nitrate loss through tile drainage water were measured in twelve plots. Nitrate concentrations in soil pore water down to 2 m depth were also measured in four of the twelve plots (fertilized and unfertilized continuous corn, and fertilized and unfertilized rotation corn). The fertilized continuous corn (CC) and rotation corn (RC) treatments produced 4-year (1999-2002) flow weighted mean nitrate concentrations in tile drainage water of 15.0 and 20.3 mg N/l, respectively, both exceeding the Canadian drinking water guideline value of 10 mg N/l. Mean nitrate concentrations in the soil pore water below tile depth were also greater than the guideline value for these two plots. Over the 4-year period, total cumulative nitrate losses in tile drainage water were large at 131.6 kg N/ha for fertilized RC and 79.7 kg N/ha for fertilized CC. In contrast, cumulative nitrate losses in tile drainage were very low for unfertilized bluegrass (4.4 kg N/ha), fertilized bluegrass (6.6 kg N/ha) and unfertilized CC (7.5 kg N/ha). Hence, crop management practices can have a profound effect on both nitrate loss from the crop root zone and nitrate contamination of tile drainage water.

WQ16 Investigation of water quality problems on the Bonnechere river system

Project Leader: Ben Hawkins
Kemptville College
University of Guelph
Kemptville ON K0G 1J0
613-258-8336 x458
bhawkins@kemptvillec.uoguelph.ca

Project Duration: May 2000 - April 2003

OASIS #: 25440

Abstract:
The project encompassed a thorough study of the Bonnechere River system in order to evaluate the impact of industry, farms and residential practices on water quality. It appears that over the years the quality of the water along this water course has slowly been deteriorating. The different stakeholders utilizing this natural resource are concerned and would like to determine the cause or causes of the problem.

The Bonnechere River system flows from Algonquin Park to the Ottawa River and its main uses include recreational, municipal water source and agricultural water source. Any contaminants introduced to this system end up in the Ottawa System. The reduction of pollutants in this water system will increase its value for recreational use and reduce the cost of treatment for municipal, and domestic use. This project investigated the factors that alter water quality as the river flows from Algonquin Park to Renfrew, Ontario.

WQ17 Evaluation of manure application machinery

Project Leader: Ben Hawkins
Kemptville College
University of Guelph
Kemptville ON K0G 1J0
613-258-8336 x458
bhawkins@kemptvillec.uoguelph.ca

Project Duration: May 2003 - April 2004

OASIS #: 25963

Abstract:
Disposal of manure produced from livestock operations has the potential of being a source of pollution for surface and ground water if not handled properly. Nutrient management planning is necessary to ensure the nutrients are being supplied at rates that do not exceed the crop requirements. The equipment available for applying the manure has to have the ability to spread over a range of application rates because of the variety of animal wastes and the management systems employed. The application rates normally based on plant nitrogen requirements now have to take into account soil phosphate levels. In many cases this reduces the acceptable application rates. Recommendations are made based on the above mentioned conditions as to the rate of nutrient application. The spreaders can deliver a wide range of application rates but, the question is, are they able to meet these recommendations?

Timing of application is also an important factor in nutrient management planning. Reducing the rate and using multiple applications can also benefit the crops being grown and their nutrient uptake.

There are many different makes, types and sizes of spreaders being used in Ontario now. The equipment is spreading a wide variety of manures. Very little information is available on the application rates the machines are capable of delivering. This study will evaluate machinery being used with respect to application rates, consistency and other operating parameters.

WQ18 (AQ) Evaluation of within-field nitrogen management units based on potential for environmental impact

Project Leader: Richard Heck
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52450
rheck@lrs.uoguelph.ca

Collaborating Researchers: I. O'Halloran, Ridgetown College; John Lauzon, Gary Parkin, Land Resource Science, Bill Deen, Plant Agriculture and Wanhong Yang, Geography

Project Duration: May 2003 - April 2007

OASIS #: 26008

Abstract:
The development of field management strategies aimed at optimizing crop yield stability pattern, within economic constraints, yet minimizing the impact of the production system on ecosystem health, requires good knowledge of the spatial and temporal variability of the soil resource. Information contained in conventional soil and crop yield-monitor surveys is frequently inadequate for site-specific applications; it may be argued that a lack of site-specific functional soil information is the major limitation to the implementation of site-specific management practices. Traditional soil and crop sampling techniques, used to determine the variability in soil nutrient and moisture status, are usually too time-consuming and expensive to permit adequate delineation of nutrient management units at the field scale. This research will develop methodologies to integrate traditional techniques with high-resolution aerial imaging systems and non-contacting geophysical sensors, as well as computer simulation models, to facilitate the identification and characterization of within-field nutrient management units designed to minimize environmental losses. Of particular interest, from the standpoint of both crop production and environmental protection, will be the fate of nitrogen from fertilizers and manures.

WQ19 Identification of N management units using organic carbon

Project Leader: Beverley Kay
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52484
bkay@lrs.uoguelph.ca

Project Duration: August 2000 - August 2003

Project #: SR9057

Abstract:
An essential element in applying sources of nitrogen (N) to variable landscapes commonly found in fields is to identify areas within the field in which the sources of N should be managed the same, i.e. identification of N management units. Nitrogen is the costliest single input and it is logical to place high priority on developing criteria to identify N management units. In addition, N applied in excess of crop requirements can contribute to contamination of ground water and may be a source of nitrous oxide, a greenhouse gas. Maps of the spatial variability in yield are being generated by many farmers but information on yield does not provide indicators of the maximum economic rate of fertilizer N required. Strips across fields comparing yield response to 0 and full rates of N application can be used to estimate the spatial variation in fertilizer N requirement along the strip but supplementary measures are required to extrapolate the data from these strips to the rest of the field. The hypothesis we propose to test in this study is that management units can initially be defined on the basis of the spatial distribution of organic carbon (OC) and that in the zones of OC content that are greater than the stable carbon content, supplementary information was required that would account for the spatial variability in yield response to nitrogen.

WQ20 (SQ, AQ) Estimating N requirements in a variable landscape from soil and climate data

Project Leader: Beverley Kay
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52484
bkay@lrs.uoguelph.ca

Project Duration: May 2003 - April 2004

OASIS #: 25977

Abstract:
Mineralization of soil organic matter (including residues of previous crops) provides a significant part of the supply of soil nitrogen (N) that is used by the growing crop. Studies have shown that the spatial variation in organic matter content can be correlated to the amount of N mineralized across a landscape. However, these studies have also shown that at some positions in the landscape, the variation in mineralization between seasons due to weather can be as large or larger than the variation across the landscape within a season. Although this means that the requirement for supplementary N fertilizer is strongly dependent on landscape position, weather and their interaction, these combined effects on fertilizer requirement have received little attention to date.

The objective of the research will be to develop and assess a methodology for predicting the amount of mineral N in the soil at the time of side-dressing in variable landscapes. The prediction will be based on the spatial variation in soil characteristics and antecedent weather conditions.

Data that has been collected will be used to identify the most effective way of using weather variables. These procedures will then be used to develop a multiple regression model that will relate pre-side-dress soil N to soil characteristics and weather variables. The model will then be extended through measurements on soils with a broader range of textures in studies off station from 2003 to 2006 and the model tested against an independent data set. If we can predict pre-side-dress-N content successfully this information will enable farmers to make a more cost-effective decision about fertilizer requirements under different weather conditions.

WQ21 Managing rural water supplies during drought conditions

Project Leaders:

Reid Kreutzwiser
Geography
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52174
reidk@uoguelph.ca

Rob de Loë
Geography
University of Guelph
Guelph ON N1G 2W1
519-824-4124 x53525
rdeloe@uoguelph.ca

Project Duration: May 2003 - April 2005

OASIS #: 26087

Abstract:
Much uncertainty surrounds water supplies in Ontario, for agricultural and other rural water users. This reflects several factors, including increasing water demands among competing users, periodic dry spells which further increase demands while reducing supplies, and questions about the effectiveness of existing water allocation arrangements. The need for drought preparedness has been recognized by the Province of Ontario, which in 2001 implemented a plan for responding to low water levels. However, important questions about this plan's ability to reduce water supply uncertainty for agricultural and rural users need to be addressed.

This research responds to this need by examining temporal and spatial trends in agricultural water use, and evaluating the prospects for effective drought management in those areas in southern Ontario where agricultural water use is high and/or increasing.

The research will proceed in two phases. In the first it will create two major deliverables: (1) estimates, mapped at the township scale, of changes in agricultural water use in southern Ontario between 1991, 1996 and 2001; and (2) an evaluation of drought management experience in the Big Creek watershed (Township of Norfolk), one of the first areas to establish a Water Response Team under the Province's drought plan. In the second, the researchers will broaden their evaluation of the drought plan implementation in selected watersheds where high and/or increasing agricultural water use was identified in part one. The research will evaluate the potential applicability of Geographical Information System (GIS) technology to enhance drought preparedness at the watershed and subwatershed scales.

WQ22 Quantifying Leaching of Nutrients and Bacteria and Amount of Preferential Flow Under Different Management Practices

Project Leaders:

John Lauzon
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52459
jlauzon@lrs.uoguelph.ca

Bill Deen
Plant Agriculture
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x53397
bdeen@uoguelph.ca

Collaborating Researchers: I. O'Halloran, Ridgetown College and G. Parkin, Land Resource Science

Project Duration: June 2002 - September 2005

OASIS #: SR9121

Abstract:
It is common knowledge that under certain soil conditions applied liquid manure can enter the tile drainage system quickly (presumably through preferential flow) and be transported to surface waters. Although this has been known for some time the number of experiments, which have attempted to measure this loss directly, are minimal. Desiccation cracks and earthworm burrows of the bio-channel are in essence large pores that can transmit liquids and the constituents in the liquids to tile drainage systems or groundwater. The extent of desiccation cracking is typically greatest in the late summer/ early fall when soil moisture contents tend to be at the lowest levels and as such it is likely that the potential for preferential flow losses will be greatest at this time period.

Disruption of the large pores by tillage is likely to reduce the potential for preferential flow. Late summer manure application also allows for a considerable amount of time for winter cover crop growth which may sequester soil and manure N reducing the potential for fall and spring leaching losses. Although the measurement of preferential flow and leaching losses will be the major focus of this research, the relative denitrification and volatilization losses will also be measured.

The project will compare the preferential flow and leaching losses of nutrients and bacteria under fields amended with liquid manure in late summer to early fall. Each plot will be situated above an existing tile drain at the Elora research station. The tile drains will then be isolated for each plot and a tile drain monitor will be installed to measure both the volume of drain effluent and the concentration of N, P, and fecal coliforms. The cost of the method of sampling dictates a limited number of treatments. Only 5 treatments will be used including; a tillage component (notill vs. conventional till with and without fall applied manure), and a spring applied manure treatment.

This may lead to reduced leaching losses of nutrients and more efficient use of the nutrient by the crop planted in the following spring. However, the environmental impacts of applying manure in fall followed with planting of a cover crop are not fully understood.

WQ 23 (SQ) A comparative study of the losses of nitrogen from various forms of manure applied either in the fall or in the spring

Project Leader: John Lauzon
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52459
jlauzon@lrs.uoguelph.ca

Collaborating Researchers: G. Parkin, Land Resource Science and I. O'Halloran, Ridgetown College

Project Duration: June 2003 - September 2006

OASIS #: SR9134

Abstract:
In Ontario, a Nutrient Management Act was passed on June 27, 2002. The intent of the act is to "enhance the protection of Ontario's water resources by minimizing the effects of agricultural practices on the environment". It establishes the requirement for Ontario producers to formulate nutrient management plans (NMPs) based on standards/best management practices (BMPs).

A nitrogen index (N-index) is included as part of the regulations in the nutrient management act. The N-index is based on the potential for loss of N to the environment (primarily losses to ground water). Within the N-index there are calculations related to how timing and type of manure applied will influence the amount of loss of N. The values in the tables are largely based on studies that considered the agronomic value of different manures applied at different times in the year and did not necessarily measure loss to the environment. To this end research is needed to confirm or adjust the predicted N losses in the N-index. The objective of the proposed research is to generate data that can be used to develop appropriate standards for application timing of hog, beef, dairy, and poultry manure. The study will examine the relative agronomic value of fall versus spring applied manure N for four different types of manure. It will also assess the impact of time of application and manure type on N losses as either ammonia volatilization or nitrate leaching. Finally, it will gauge the impact of pre-application tillage on manure N losses from late-summer applied manures.

WQ24 Construction of recombinant antibodies for detection of Cryptosporidium parvum

Project Leader: Hung Lee
Environmental Biology
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x53828
hlee@uoguelph.ca

Collaborating Researchers: J. Trevors, C. Hall, Environmental Biology

Project Duration: May 2000 - April 2003

OASIS #: 25562

Abstract:
The enteric protozoan Cryptosporidium is a widely distributed pathogen causing the gastrointestinal illness cryptosporidiosis in both humans and several species of farm animals. Water is a common vehicle for transmission of Cryptosporidium spp. In the past few years, several waterborne outbreaks of cryptosporidiosis have occurred in Ontario due to run-offs from agricultural fields or human fecal contamination. Although different species of the parasite exist, Cryptosporidium parvum appears to be the most widely distributed and commonly implicated in clinical disease outbreaks. Reliable detection of Cryptosporidium parvum is essential for routine monitoring and prevention of cryptosporidiosis.

This project was a collaborative study that was designed to construct recombinant antibodies against C. parvum. If successful, the researchers will have available a ready source of cheap bacterial recombinant antibodies that can be used for the specific, sensitive and effective detection of C. parvum in environmental samples. At this point the project has successfully immunized mice and generated combinational libraries of scFV fragments from splenocytes of the immunized mice. Unfortunately, the project has thus far been unsuccessful in selecting a suitable recombinant antibody against C. parvum oocysts. Researchers plan to continue screening for the recombinant antibody as it may ultimately lead to the safeguarding of rural drinking water supply.

WQ25 Waste signaturing: Evaluating the utility of stable isotope ratios for the identification of aquaculture effluent in the natural environment

Project Leader: Richard Moccia
Animal and Poultry Sciences
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x56216
rmoccia@uoguelph.ca

Project Duration: May 2003 - April 2005

OASIS #: 26053

Abstract:
Continued growth of Ontario's cage-aquaculture industry is constrained by the need to manage, and sometimes mitigate, the environmental impacts resulting from nutrient waste production from these farms. It is extremely difficult to identify the source of impacts in a dynamic environment where these nutrients are found naturally and can also be contributed to by other anthropogenic inputs. One method that shows promise to validate existing monitoring practices, assist in mitigation, and determine the source of nutrients is the use of stable isotope ratios (SIRs) to define an aquaculture 'waste signature'. Elements of biological interest such as hydrogen, carbon, nitrogen and oxygen have two naturally occurring isotopes. The ratio of heavy to light isotopes in a sample may be able to specify the samples' origin. This approach has been successful in tracking sewage through aquatic environments, but has only recently been considered for aquaculture.

Ultimately, the use of stable isotopes to track net-pen nutrient loading will require testing in the Great Lakes where most of the province's fish production occurs. However, prior to initiating such large-scale field trials, it is prudent to test the analytical success and the statistical significance of isotopic ratios in a controlled laboratory setting. The result of this research will dictate the approach to future trials in the Great Lakes. The isotopic ratios of carbon, nitrogen, oxygen and sulfur will be determined in samples of fish feed, inflow, fecal mater and tank effluent of cultured rainbow trout.

WQ26 Validation of operational modeling and monitoring (VOMM) of nutrient outputs from cage-based aquaculture in the great lakes

Project Leader: Richard Moccia
Animal and Poultry Sciences
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x56216
rmoccia@uoguelph.ca

Project Duration: May 2000 - April 2003

OASIS #: 25320

Abstract:
Continued growth of Ontario's cage-aquaculture industry is constrained by the need to manage, and sometimes mitigate, the environmental impacts resulting from nutrient transport from these farms. Farmers, regulatory agencies and special interest groups are demanding credible and quantitative methods of monitoring this nutrient transport, so that farm production practices can be sustainable over the long-term, by operating within the assimilative capacity of the affected watershed. Currently, water quality monitoring programs are expensive and do not permit either the quantification of nutrient enrichment, or the prediction of deleterious eutrophication effects. As a consequence, cage aquaculture operates in a tenuous and sometimes volatile regulatory climate which can impact the success of the farming system.

The aim was to develop credible, quantitative and predictive methods of monitoring the nutrient transport from fish-farms in open water systems in the Great Lakes. The key element of the study involved the validation of innovative, continuous water quality monitoring technology, followed by the integration of the monitoring results into a predictive computer model. This 'predictive' model will help estimate expected nutrient loads and transport from cage-aquaculture farms, and will provide the basis for a rational, regulatory compliance system based on sound, empirical observations. The information will also help predict the onset of eutrophic and/or hypoxic events which are the key environmental effects which must be avoided if aquaculture is to survive in the Great Lakes watershed. Early warning systems such as these are necessary to avoid, rather than require rehabilitation of, negative environmental effects.

WQ27 Neural networks for predicting nitrate - nitrogen in drainage water

Project Leader: Satish C Negi
School of Engineering
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52231
scnegi@uoguelph.ca

Collaborating Researcher: R. Rudra, School of Engineering

Project Duration: May, 2001 - April, 2003

OASIS #: 25595

Abstract:
The potential for pollution of groundwater by nitrate from the use of manure and fertilizers is a major concern in both Canada and the U.S. A recent study of tile effluent from manured and fertilizer cornfields near Ottawa indicated that a significant number of samples exceeded the 10 mg/l nitrate-nitrogen limit for drinking water. The information gathered during the course of this study included details on soil, daily climate, agricultural management practices, drain flow, nitrate concentration and loss in tile effluent. The data will be used for training two artificial neural networks (ANNs) to predict nitrate-nitrogen concentrations in subsurface tile effluent. ANNs can learn from the relationship between input and output from experiments and are capable of generalizations based on the knowledge that has been gained. Two distinct types of ANNs will be evaluated: (1) the trainable fast back-propagation (FBP) network, and (2) the self-organizing radial basis function (RBF) network.

Using the algorithms of FBP and RBF networks, a software package comprising of modules will be developed to reduce the network training time and increase its flexibility. The available field data will be divided into training and testing scenarios, with the training file consisting of seven inputs and two outputs. A sensitivity analysis will be performed by varying the network parameters to minimize the prediction error and determine the optimum network configuration. The performance of the two networks will be compared with that of a conventional model (DRAINMOD-N). The recommended neural network can be used for assessing the impact of the practice of nitrogen application, either from animal sources or fertilizer, on groundwater quality.

WQ28 Assessing manure phosphorus bioavailability and potential for loss

Project Leader: Ivan O'Halloran
Ridgetown College
University of Guelph
Ridgetown ON N0P 2C0
519-674-1635
iohallo@ridgetownc.uoguelph.ca

Project Duration: May 2003 - April 2005

OASIS #: 26046

Abstract:
Previously, manure applications to agricultural lands were based primarily, if not entirely, on the nitrogen (N) requirements of the crop. The imbalance between a crop plant's requirements for N, phosphorus (P) and potassium, and the relative proportion of these elements in livestock manure results in the potential for soil build up of phosphorus and potassium. Provincial monitoring stations indicate that some 50% of Ontario's watersheds still exceed the Provincial Water Quality Objectives for P. Non-point source of pollution, both agricultural and urban, generally contribute between 70-90% of the total P load. Considerable effort has been placed on the development of nutrient management plans for farmers that would address public concerns regarding the production, storage and use of agricultural nutrients.

An environmental risk assessment tool, the P index, was developed to help producers identify potential problems and appropriate management practices to reduce the risk of P transport from the field via overland flow/erosion. Recent studies have indicated that aspects regarding manure P not specifically addressed in the P index may play an important role in determining the potential for P to move from a field or the subsequent impact of manure applications on soil test P levels and plant P uptake. This project deals with aspects of the P-index such as methods of manure handling and analyses to determine the nutrient availability and potential risk of loss of P contained in manure and the residual availability and contribution of this manure-P to soil test P levels.

WQ29 Impact of management strategies to lower P excreted in manure on the bioavailability of P in manure and manure-amended soils

Project Leader: Ivan O'Halloran
Ridgetown College
University of Guelph
Ridgetown ON N0P 2C0
519-674-1635
iohallo@ridgetownc.uoguelph.ca

Collaborating Researcher: M. Goss, Land Resource Science

Project Duration: May 2001 - April 2003

OASIS #: 25669

Abstract:
Provincial monitoring stations indicate that some 50% of Ontario's watersheds exceed the Provincial Water Quality Objectives for P. The imbalance between a crop plant's requirements for nitrogen (N) and phosphorus (P), and the proportion of these elements in manures increase the potential for soil P build up when manure applications are based only on N requirements. The excess P in manure from monogastric animals (e.g. hogs) can be linked to the fact that the dominant form of P in corn and other feed grains is phytate-P, a form that is relatively unavailable to these animals. Addition of mineral-P supplements to the animal's ration can also contribute to higher manure P levels. Three possible methods to deal with this manure-P problem involve the: i) use of low phytate feed sources, such as low phytate corn; ii) addition of phytase enzymes to the ration which increases the utilization of phytate-P by the animal; and iii) genetic modification of the animal so it will produce it's own phytase enzyme (i.e. Enviropig). The influence of these management strategies on manure P bioavailability was compared to animals fed a normal feed ration, with and without a mineral P supplement. Manure from animals fed a mineral P supplement had considerably greater levels of total and readily available forms of P. Of the strategies developed to improve the utilization of P in the feed by the animal, the Enviropig produced the manure with the lowest total P contents. In addition, compared to the non-transgenic pig fed the same diet, the Enviropig™ reduced the proportion of P found in the moderately available inorganic and organic fractions, while increasing the amount of P found in very resistant fractions. The implications of these changes in manure P fractionation in terms of plant availability and risk of P movement after soil application are still under study.

WQ30 Quantifying preferential flow and recharge at the field scale: The first step in characterizing the nature and timing of pathogen transport to groundwater

Project Leader: Gary Parkin
Land Resource Science
University of Guelph
Guelph, ON N1G 2W1
519-824-4120 x52452
gparkin@lrs.uoguelph.ca

Project Duration: October 2001 - October 2004

Project #: SR9084

Abstract:
In a recent survey of water wells in rural Ontario it was discovered that approximately one-third were contaminated with coliform bacteria. A recent Ontario study has shown that water flow through cracks and macropores in soil can transport bacteria 100 metres or more from sources such as septic systems and manure storage facilities. This research project will quantify the amount of water that flows preferentially through soil on an annual basis. It will document the seasons during the year when most preferential flow occurs and how the amount of deep drainage is affected by position on a sloping landscape and distance to tile drains. Innovative methods will be used to measure the quantity of deep drainage that occurs through macropores such as cracks and worm burrows in the soil. Automatic tile flow and groundwater level monitors and water sampling systems will be used to determine how much water reaches tile drains or the water table preferentially during rain and snow melt events throughout the year. It will also help to define during which season(s) drinking water wells will be most susceptible to contamination from bacteria. Results from this research will assist in determining where best on the landscape to install instruments to measure the field-average recharge and potential leaching losses of contaminants from agricultural sources. This is invaluable information for groundwater protection schemes which can be of significant economic benefit to Ontario.

WQ31 Quantifying influence of management practices, tile drainage on soil chemical and moisture dynamics using non-intrusive geophysical methods

Project Leader: Gary Parkin
Land Resource Science
University of Guelph
Guelph, ON N1G 2W1
519-824-4120 x52452
gparkin@lrs.uoguelph.ca

Collaborating Researcher: R. Heck, Land Resource Science

Project Duration: May 2001 - April 2003

OASIS #: 25652

Abstract:
There is a need to develop and evaluate techniques for the identification, assessment and control of significant sources of point and non-point source pollution from the agri-food system to direct application of best management practices. Fields with subsurface drainage tiles present a considerable challenge for application of agricultural chemicals and organic wastes, to minimize both leaching losses and subsequent contamination of waterways. This arises from the variability introduced into the rate of water flow through the soil and its impact on basic chemical processes. Detailed geo-referenced surveys of soil electrical conductivity and magnetic susceptibility (using a non-contacting magnetic induction probe) and soil moisture content (using ground penetrating radar), offer a means of delineating soils within agricultural fields according to water regime and, therefore, basic chemical properties. In particular, measurement of the width of the zone of relatively rapid drainage above a tile drain will be valuable information for designing site specific management practices to reduce nutrient and pathogen losses when spreading liquid manure on tile-drained fields.

This project aimed to evaluate basic soil moisture dynamics together with key chemical characteristics of the soil, in relation to drainage tiles that have been in place for about 30 years and a recent change in management practice from tillage to no-tillage. Moisture flow, chemical and pathogen transport through the soil is often via larger pores, therefore analysis of the short-range spatial variations in chemical composition of soils adjacent to macropores was also carried out on intact soil samples. This information will be used to understand the delineations identified with field geophysical instrumentation.

WQ32 Assessment of the waterborne pathogen abatement effects for agricultural land uses

Project Leader: Ron Pushchak
Occupational and Public Health
Ryerson University
Toronto ON M5B 2K3
416-979-5000 x7049
pushchak@ryerson.ca

Collaborating Researchers: S. Liss, Faculty of Engineering and Applied Science

Project Duration: July 2002 - June 2004

Project #: SR9118

Abstract:
This research examines the effects of nutrient management measures on agriculturally generated pathogens, their survival and transport. These measures, while not explicitly described as pathogen control actions, raise the expectation that pathogen survival and transport will be reduced if nutrient management regulations are put into practice. An understanding of the measures and their effectiveness require a study with two elements. The first is an investigation of the scientific bases for the current nutrient management regulations in Ontario, with an examination of the broader context of nutrient management policies in other jurisdictions where agricultural practices make nutrient management regulations necessary. The second is an analysis of the pathogen survival and transport outcomes of the proposed regulations drawing on the scientific evidence available for a number of indicator organisms.

The key features of the research include: i) a comprehensive review of the nutrient and pathogen management policies and regulations applied to agricultural operations in a number of jurisdictions; ii) an analysis of the Ontario nutrient management regulations and their potential pathogen control outcomes, and specifically, the likely survival and transport of indicator pathogens in Ontario rural environments, assuming the implementation of the regulations; iii) identification of potential limitations of the regulations to control pathogens and a review of further measures that agricultural operators of nutrient sources may take to reduce pathogen exposure risks in surface and groundwaters; iv) training of research students in the area of environmental health protection where the integration of applied science and policy is becoming increasingly important.

WQ33 Development of watershed system capacity procedure for water quality

Project Leader: Ramesh Rudra
School of Engineering
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52110
rrudra@uoguelph.ca

Collaborating Researchers: M. Goss, G. Parkin, Land Resource Science; R. Corry, School of Environmental Design & Rural Development; P. Sibley, Environmental Biology

Project Duration: May 2003 - April 2005

OASIS #: 26028

Abstract:
This project will provide tools to develop water quality system capacity (TMDL) to identify any impaired water bodies in the watershed, select best management practice for improving the impairment and evaluate the effect of climate change on the water quantity and quality. It will also provide the geo-reference (location) of the pollutant sources (point and non-point) and their relative contribution to the impairment of water bodies in the watershed. A procedure for the selection of BMP's to improve water quality under change in climatic scenarios will be developed. This project will also provide an assembled database for the improvement of watershed management tools and for future model improvement, BMP evaluation, and MDL development. The project will help consultants involved in watershed management and TMDL implementation policies. The beneficiaries will be the agricultural (rural) community, land users, and other stakeholders affected by the impact of agricultural land use activities on rural water quality.

WQ34 Design tools for riparian vegetative filter strips incorporating characteristics of the contributing watershed

Project Leader: Ramesh Rudra
School of Engineering
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52110
rrudra@uoguelph.ca

Collaborating Researchers: M. Goss, G. Parkin, Land Resource Science; P. Sibley, Environmental Biology and H. Whiteley, School of Engineering

Project Duration: May 1999 - April 2003

OASIS #: 23870

Abstract:
In Ontario riparian buffer strips are recommended to protect and enhance the quality of stream ecosystems in agricultural areas. The value of riparian ecotones for enhancement of streams and as a valuable landscape element in their own right has been examined extensively in general terms elsewhere but data is lacking for Ontario, especially in establishing criteria for optimum width and vegetative type for riparian buffers based on the soil, land use and topographic characteristics of the contributing areas. The design and performance of vegetative filter strips for treatment of runoff from cropland depends on the quality of estimation of runoff, sediment and nutrient loads from upland areas which is commonly done by application of non-point source pollution models. A computer-based management tool was developed for the design of vegetative filter strips based on the characteristics of the upland contributing area. A practical management tool for the design of vegetative strips was developed by combining an existing agricultural non-point source pollution model, modified with a vegetative filter strip model, for application in Ontario conditions. Site specific recommendations for the width and type of vegetation in the buffer strips will be made by application of the combined model.

WQ35 (AQ, SQ) Advanced manure management technologies for Ontario (AMMTO)

Project Manager: Richard St. Jean
(519) 886-7500 x225
rstjean@geomatrix.com

Project Duration: October 2001 - December 2003

Healthy Futures #: 2198

Abstract:
This project was initiated to develop a decision making process and tools to be used by livestock producers and regulators to determine the viability, capabilities and limitations of manure management systems and technologies for Ontario livestock farms. The decision-making tools developed include: a standard Manure Management Technology Information Request Form, an Economic Evaluation Template, a Steps to Implement Database and an Evaluation Process. The report will be available on the Internet so that it is accessible, searchable and updateable for all producers and regulators.

WQ36 Removal efficiency of representative bacterial and protozoan pathogens from cattle and pig farm wastewater using wetland technologies

Project Leaders:

Robin Slawson
Department of Civil
Engineering
University of Waterloo
Waterloo ON N2L 3G1

Barry Warner
Department of Civil
Engineering
University of Waterloo
Waterloo ON N2L 3G1
bwarner@waterserv1.uwaterloo.ca

Project Duration: January 2002 - December 2004

Project #: SR9070

Abstract:
Wetland technology is emerging as a cost-effective and environmentally friendly option for treating wastewater and improving water quality. Organic-rich agricultural wastewater has been shown to be well suited to treatment by wetland technology. The ability of wetland technologies to remove pathogens from agricultural wastewater is less well known, although research on the removal of pathogens from domestic wastewater by wetlands gas been shown to be effective.

This project aims to monitor the wetland removal efficiency for pathogenic enteric bacteria (Escherichia coli, Salmonella, Campylobacter), and protozoa (Crytosporidium) in representative subsurface flow treatment wetlands systems on a cattle and on a pig farm in southwestern Ontario.

The primary objective of this project is to characterize the spatial distribution and year round temporal variability of pathogenic enteric bacteria (Escherichia coli, Salmonella, Campylobacter), and protozoa (Crytosporidium) on soil particles, in the soil water and on the plant roots (rhizosphere). Wastewater from a pig farm versus a cattle farm will be compared for efficiency of the wetland to remove these pathogens. A network of sampling points, both shallow and deep, will be setup in each of the wetland cells operating at each of the farm sites. Monthly sampling will be undertaken. This two-level factorial design will aid in assessing the significance of plant root material in microbial establishment and pathogen removal, as well as the influence of seasonal temperature on the population development.

This project will contribute data that will aid in our understanding of the removal efficiency of pathogens by wetland technologies that will assist in the refinement and parameterization of improved wetland designs in the future. Further, it is hoped that this work will contribute to greater understanding and acceptance of wetland technologies as a viable, inexpensive, and "green" wastewater treatment option, in particular for pathogen removal, in the agricultural community. This work will have considerable economic benefit by contributing to effective low-cost wastewater treatment alternatives to current BMPs and for watershed and source water protection for farmers and rural residents.

WQ37 DNA fingerprinting analysis of Escherichia coli using repetitive element and toxin specific gene amplification to differentiate pollution sources in the environment

Project Leader: Michele Van Dyke
GAP EnviroMicrobial Services
1020 Hargrieve Road, Unit 14
London, ON, N6E 1P5
519-681-0571
mvandyke@gapenviromic.com

Collaborating Researchers: A. Scott, University of Guelph and J. Crawford, GAP EnviroMicrobial Services

Project Duration: May 2001 - August 2002

Project #: SR9071

Abstract:
The presence of pathogenic bacteria in potable, agricultural and recreational water supplies can have serious consequences. To effectively control inappropriate releases of fecal waste to the environment, methods to determine the point source of origin should be available.

Escherichia coli is a useful indicator of fecal contamination. The researchers have used this strain together with methods for subspecies analysis. In this project, the researchers attempted to determine if individual subspecies of E. coli could be traced to a specific type of animal host. The analysis could then be used to differentiate between point sources of origin of fecal waste.

A number of methods are available for subspecies analysis but molecular biological methods showed the greatest promise. Molecular methods are able to distinguish between closely related bacteria by producing DNA patterns or fingerprints that are specific for individual strains. Often one method alone does not produce sufficient complexity to allow animal sources to be identified. For this reason, the researchers used a combination of methods.

The methods researchers used for this analysis included, DNA amplification of repetitive-element sequences (rep-PCR) and amplification of specific toxin genes. Each of these methods has shown promise in differentiating between E. coli strains. Preliminary research suggested that individual methods may be able to distinguish between animal sources of origin.

Results of this project produced a statistical analysis of banding patterns produced from E. coli strains. The analysis showed that there was poor separation when grouped by individual host animals. Using the group violations analysis, it was found that there was a large amount of overlap between the groups. It was concluded that the group separation values were not sufficiently high to allow sensitive discrimination of an animal host. Rep-PCR analysis was also performed on 50 Ent. faecium isolates from different sources. The ability of Rep-PCR to assign Ent. faecium isolates to individual animal sources was poor. However, the category separation of animals versus birds was high, resulting in values of 78 and 77%, respectively. Overall, researchers found that E. coli contamination could be successfully tracked from the point of origin through different watershed systems (from site studies). This analysis showed that there is potential for using Rep-PCR fingerprinting techniques in defined environments where access to potential sources is available

WQ38 (SQ) Greenhouse gas and ammonia emissions during storage, treatment and land application of animal manure

Project Leader: Claudia Wagner - Riddle
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52787
criddle@lrs.uoguelph.ca

Collaborating Researchers: R. Fleming, Ridgetown College; P. Voroney, J. Warland, Land Resource Science; H. Zhou, School of Engineering and C. Chong, Plant Agriculture

Project Duration: May 2003 - April 2007

OASIS #: 26075

Abstract:
Current knowledge of non-hazardous organic waste handling systems presents a gap in the understanding of the gaseous losses that can occur during storage, treatment and during field application. The nutrient value of the wastes for plant production and their benefits to soil can be decreased substantially due to gaseous losses during any of these handling stages. The environment also is affected by these losses, since they can occur as greenhouse gases, which contribute to climate change, and as ammonia, which contributes to eutrophication and soil acidification of natural ecosystems. This project will quantify gaseous losses from enhanced organic waste handling systems used for treatment of liquid swine and liquid dairy cattle manures, aerobic composting and anaerobic digestion, compared to storage and direct land application. The gases to be monitored are: CO2, CH4, N2O, NH3 and N2. Initial experiments will optimize parameters controlling the aerobic composting and anaerobic digestion processes to minimize greenhouse gas production and maximize NH3 retention. Enriched 15N tracer techniques of gaseous products will be used to identify and quantify each of the forms of manure N lost during storage, treatment and land application. A micrometeorological mass balance method will be used during field experiments set-up to monitor losses in-situ using tunable diode laser trace gas analyzers for gas concentration measurements. The results of this project will contribute to the environmental impact assessment of organic waste handling systems, and will provide information on how to manage these wastes to maximize their nutrient value for crop production and to soil quality.

WQ39 (AQ) Best management practices for field crops: mitigation of greenhouse gases and adaptation to climate change

Project Leader: Claudia Wagner - Riddle
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52787
criddle@lrs.uoguelph.ca

Collaborating Researchers: J. Warland, G. Parkin and T. Gillespie, Land Resource Science

Project Duration: May 2003 - April 2004

OASIS #: 26076

Abstract:
Adoption of BMPs has the potential to lead to carbon reduction credits, and can present other co-benefits such as improved water quality and conservation, uptake of air pollutants, and higher economic returns, through increased nitrogen and water use efficiency. Quantification of the benefits incurred from adoption of BMPs requires long-term monitoring of the combined effects of management strategies upon the carbon, nitrogen, water and energy budgets. The research will compare the water and nitrogen use efficiencies of a corn/soybean/wheat rotation under BMPs in comparison to conventional practices. This experiment will increase our understanding of the performance of BMPs under a range of weather conditions. Treatments will include inorganic N fertilizer and dairy manure applications, according to soil N test (BMP) and recommended rates (CP). Environmental losses in the form of NO3- leaching, gaseous N2O, NH3 and NOx will be monitored, and compared to total N losses as determined by the use of N tracers. Monitoring of the water and energy budgets will allow for determination of the water use of BMPs versus conventional practices, addressing the current need for research on climate change adaptation.

WQ40 Identification of critical regions for water quality monitoring with respect to risk of seasonal and annual water surplus

Project Leader: Claudia Wagner-Riddle
Land Resource Science
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x52787
criddle@lrs.uoguelph.ca

Collaborating Researcher: G. Parkin, Land Resource Science

Project Duration: May 2001 - May 2003

OASIS #: SR9083

Abstract:
Surplus water is the component of precipitation that runs off the land surface and that drains through the soil profile to the ground water table. This project assessed the risk of occurrence of surplus water in two Ontario climate regions with contrasting winter precipitation regimes. The two regions to be studied were- (1) the snowbelt region east of Lake Huron, e.g. Walkerton, where there is a high risk of surplus water; and (2) the Rainy River district, where there is much less winter precipitation. The study added to a recent evaluation of surplus water for Harrow, Guelph, Ottawa, and Kapuskasing regions. A one-dimensional, deterministic model was used that simulates water flux into the soil, run-off, and includes plant uptake of water, evapotranspiration, and freeze-thaw conditions. The model was applied to historical, daily climate data collected over the last 30 to 40 years at one or more weather stations in each of the two regions. The climate data was assembled from the Environment Canada archives and any missing data was obtained from surrounding weather recording stations to establish a complete set of daily climate data for input into the model.

It is anticipated that the guidelines/legislation that are being developed for application of animal wastes and sewage sludge may have to take into consideration the risk of occurrence of water surplus events in the different climate regions of Ontario. This project should provide information on the different risk levels for these regions, and perhaps for different soil landscapes within each region.

WQ41 Phosphorus index validation and refinement for nutrient management planning in Ontario

Project Leader: Greg Wall
The Soil Resource Group
Guelph ON N1H 6T9
519-837-1600

Project Duration: June 2002 - June 2004

Project #: SR9122

Abstract:
Nutrient management planning and implementation facilitates optimum use of fertilizer and manure nutrients for crop production while reducing potential impacts on environmental quality. In Ontario, the OMAF-NMAN series of nutrient management computer programs have been developed to assist producers in preparing nutrient plans. The Phosphorus (P) Index has been incorporated into the NMAN program to: rank risk of water contamination, set restrictions on P application rates and determine the distance that P applications must be set back from water courses. Ontario's P index was adapted from the procedure described by Lemunyon and Gilbert (1993) for the USA. The coefficient in the index has not yet been calibrated or validated against actual water quality data in Ontario. Recent research in the USA to improve P indexing procedures has proposed both additional coefficients and revised weighting of coefficients.

The results of the study will provide a scientifically defensible P index for the planned provincial nutrient management program. Producers will benefit from a P indexing procedure that offers greater flexibility managing manure and fertilizer nutrients.

The specific objective of this study is to provide a science-based validation of the Ontario P index with measured soil P and water quality (TP and DP) data. Further, alternative coefficients or weightings of coefficients for P indexing found in recent or ongoing research will be evaluated for use in the Ontario NMP procedure.

Study data will be used to validate the Ontario P index being used in the NMAN nutrient management program and recommend modifications in coefficient weightings that are supported by the database. Further, the database will be used to evaluate new coefficients and coefficient weighting schemes that are being proposed in ongoing research on P indexing in the USA.

Expected outcomes of the study are a revised P indexing procedure that reflects the current state of knowledge and that has been validated with Ontario water quality data.

WQ42 Protecting water quality through improved storage methods for poultry manure

Project Leaders:

Greg Wall
Soil Resource Group
Guelph ON NIH 6T9
519-837-1600
gwall@agtest.com

Don King
Soil Resource Group
Guelph ON N1H 6T9
519-834-1600

Collaborating Researchers: H. Fraser, OMAF and D. Nodwell, Poultry Industry Council

Project Duration: July 2000 - July 2002

Healthy Futures #: 2059

Abstract:
This two year applied research project quantified liquid runoff volumes from dry poultry manure (>50% dry matter) stored outside and used in agricultural crop production, and assessed its quality and risk to the environment under a variety of conditions in a replicated experimental procedure. Recommendations were developed for cost-effective, practical methods that both satisfy the needs of producers, while protecting the environment and rural public. Chicken broiler (straw and shavings bedding) and Tom turkey manure (shavings bedding) were selected for study in on-farm situations.

Stockpiles were monitored over winter (October to April) and summer (June to September) storage periods. Runoff quantity and quality were measured from rainfall events over the storage period. Runoff events at the start and end of the storage period were created with a rainfall simulator that generated rainfall events ranging from a 2 to 200 year return-period storm. Variable conditions of the pile at the commencement of rainfall were tested, including dry and damp piles under warm and cool conditions.

Monitoring included runoff volume and quality (nutrients and bacteria), pile temperature, pile settling, nutrient and moisture content.

Runoff from stockpiled poultry manure was generated in summer and winter storage periods from small rainfall events (< 5-yr return period storm). While runoff volumes were low, the nutrient and bacteria concentrations in the runoff were high relative to water quality standards. Direct seepage beneath poultry piles was not generated within a single season storage period. Remedial practices that keep stockpiled poultry manure dry or retain any runoff on-site are therefore warranted to minimize potential impacts of runoff on surface or subsurface water quality. Pile temperature observations suggest that the risk of combustion (fires) can be minimized by keeping the stockpiled manure dry or avoiding stockpiling dry material on top of wet manure material. Several temporary cost-effective remedial practices including: pile shaping methods, tarp covering options, an absorbent ring or berming around piles were demonstrated to reduce runoff and improve water quality.

WQ43 Economic analysis of conservation cover programs and best management practices

Project Leader: Alfons Weersink
Agricultural Economics & Business
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x53532
aweersin@uoguelph.ca

Collaborating Researchers: G. Fox, Agriculture Economics & Business and W. Yang, Geography

Project Duration: May 2003 - April 2007

OASIS #: 26023

Abstract:
Growing concern about the adverse environmental effects of agriculture has led to calls for the establishment of conservation stewardship programs in Canada. Programs such as the Greencover program and the Rural Water Quality Program could generate environmental and economic benefits to our society that outweigh the program costs (Belcher, et al., 2002). However, given limited government budgets and the heterogeneity of eligible land parcels, an important policy question is how to target land (or practices) selectively in order to maximize program benefits and minimize program costs.

A framework that combines economic, hydrologic and GIS modeling will be developed to examine cost-effective targeting of conservation cover programs and best management practices in agricultural watersheds. Within the framework, farm-level economic models will be constructed to quantify the costs of implementing conservation practices. A hydrologic model will be used to estimate water quality benefits that could be achieved through implementing conserving practices. A GIS-based mathematical modeling system will be used to integrate economic and environmental data and to simulate targeting scenarios. The framework will be applied to several representative watersheds in Ontario starting with the Canagagigue subwatershed in the Grand River Basin.

WQ 44 Use of chemical chelation and membrane filtration technologies to control phosphorus release from recirculation aquaculture systems.

Project Leader: H. Zhou
School of Engineering
University of Guelph
Guelph ON N1G 2W1
519-824-4120 x56990
hzhou@uoguelph.ca

Project Duration: May 2003 - April 2004

OASIS #: 26105

Abstract:
Due to growing environmental concerns over water quality impacts, limited availability of water resources, increased costs of effluent treatment and monitoring, and a trend towards production of warmer-water fish species in Ontario, recirculating aquaculture systems (RAS) are increasingly being employed by the provincial aquaculture industry. However, elevated fish densities in a limited aquatic space, coupled with a very high percentage reuse of the water supply, leads to a more severe deterioration of the final effluent discharge water quality than occurs in more conventional, 'flowthrough' types of systems. Some of the reported problems include elevated concentrations of suspended solids (SS), total phosphorous (TP) and nitrogen (N), as well as other organic materials. In particular, phosphorus levels greatly exceed the enforced regulatory limit for effluent discharge, creating environmental compliance problems because although the total volumes of waste-water are greatly reduced, the absolute concentrations of nutrients can be very high. As a consequence, RAS technologies can create regulatory issues with effluent chemical quality, preventing compliance with the 'Certificate of Approval', the current legal instrument in Ontario controlling wastewater discharge from most land-based fish farms. Hence, effective treatment required to maintain high water quality for fish production, as well as to meet the standard of strict environmental regulations becomes critical for the future growth of aquaculture industry.

The aim of this project is to investigate the effectiveness of combining hybrid membrane filter technologies with chemical chelation for effecting phosphorus removal, in order to achieve regulatory compliance of the final effluent discharges from RAS aquaculture systems.

The primary benefit of this research is that it may assist with the regulatory compliancy of RAS technologies, and allow their increased integration into the industry in Ontario. The results of this research may provide the aquaculture industry with a more efficient, cost effective wastewater treatment technology based on membrane and chelation technologies. As well, the limitations, design guidelines and operating protocols of their applications will be summarized. As recirculating aquaculture systems become increasingly important to the future growth of industry, development of new membrane technologies will not only improve the fish production, but also reduce wastewater generation via water reuse.

As well, this study will also provide a better understanding of membrane fouling mechanisms when used for aquaculture wastewater treatment. At present, little information is available about the physico-chemical and biological interactions underlying membrane fouling.

The project will build on ongoing research efforts using the membrane filtration technologies for municipal wastewater treatment. None of them is directed to the treatment of effluent from the aquaculture industry.


For more information:
Toll Free: 1-888-466-2372 ext. 64554
Local: (519) 826-4554
E-mail: research.omafra@ontario.ca
Author: Paul Brine - Research Analyst/OMAFRA
Creation Date: 27 September 2005
Last Reviewed: 23 Septemer 2009